Based on the excellent flexibility of the novel lightcycler 480 software version 1. Aug 06, 2015 roche lightcycler 480 ii real time pcr system for 96 well plates 8856 2. Comprehensive suite of tools for setup, run, and analysis of realtime pcr quantification and. The lightcycler 480 ii with led is suitable for detection systems with different probe types. The system features the lightcycler 480 instrument, a versatile, platebased realtime pcr device that supports mono or multicolor applications, as well as multiplex protocols. Based on a comprehensive improvement of the peltierbased technology incorporated in a silver block, the instrument provides extraordinary welltowell temperature homogeneity and maximized interwell, intercycle reproducibility. Realtime ready custom panels create your specific gene expression panel with realtime ready assays of your choice on lightcycler 480 multiwell plates 96 or 384, using the realtime ready configurator. Primerdesign ltd covid19 genesig realtime pcr assay. The lightcycler 480 system is a highperformance, medium to highthroughput pcr platform 96well plates that provides various methods for gene detection, gene expression analysis, genetic variation analysis, and array data validation. This roche lightcycler 480 ii 384 pcr instrument is in good working order. Find and purchase roche lightcycler 480 products at invitrogen life science technologies.
Roche lightcycler 480 ii real time pcr system for 96 well. Lightcycler software roche, free lightcycler software roche software downloads, page 3. I can recommend this instrument, especially in lc480 ii version. V license statements for the lightcycler 480 gene scanning software parts of the software used for the lightcycler 480 system are licensed from idaho.
This product is covered inpart by us 5,871,908 or any foreign equivalents, coexclusively licensed from evotec oai ag. It enables users to quickly customize the system to meet different application requirements, from basic manual analysis, a wide choice of flexible quantification methods in line with miqe guidelines, to tools for multiple plate analysis. Here is a roche lightcycler 480 ii 384 pcr w thermocycler 384s predecessor to. The lightcycler 480 ii is a realtime pcr cycler for mid to high sample throughput. Roche life science realtime ready overview lightcycler. Lightcycler 480 realtime pcr system from roche applied. Realtime ready assays are available as single assays for the selected target or preplated on lightcycler 480 multiwell plates 96 or 384. This lightcycler was tested on a windows xp 32bit operating system with lc 480 software. The lightcycler 480 instrument ii 96 to 384well throughput, versatile for experienced users the lightcycler 480 instrument is a highperformance, flexible throughput qpcr platform with interchangeable 96 or 384well blocks that provides various methods for gene detection, gene expression analysis, genetic variation analysis, and. Maybe the downside is the lightcycler software that is somehow outdated and not so easy to.
The following instructions are based on lightcycler 480 software version 1. Has anybody used the light cycler 480 system by roche. Lightcycler 480 instrument ii roche applied science. Pcr device for qualitative or quantitative detection of nucleic. The user can also choose which samples to include in the display and analysis e. Parts of the software used for the lightcycler 480 system are licensed from idaho technology inc. Flexibly switch between plate formats and thermal block cyclers in just a few minutes with no recalibration time, and benefit from additional userfriendly software. Before the grouping is performed, the melting curve raw data are processed in three steps. Unfortunately, i then found out that the lightcycler480 software cannot do an. The roche lightcycler 480 rtpcr system combines amazing speed with exceptional accuracy and will meet the needs of a broad range of scientific applications in genomics research including array validation, geneknockdown studies, and snp analysis. Enter the username and assigned password to log in to the lightcycler 480 software. Lightcycler software 4 software free download lightcycler. Here is a roche lightcycler 480 ii 384 pcr w thermocycler 96s predecessor to.
Turn on the computer linked to the instrument and log on to window xp. The we are doing a walk around of the roche lightcycler 480 ii 384 pcr instrument showing connections and serial tag. Rapid highthroughput, platebased realtime pcr amplification and detection instrument. Roche lightcycler 480 ii real time pcr system for 96 well plates 8856 2. The lightcycler 480 realtime pcr system roche applied science brings you one more breakthrough in the realtime pcr analysis of gene expression and genetic variation roche applied sciences cuttingedge gene analysis technology is now available in a highthroughput, microwell platebased cycler format 96 or 384well. The lightcycler 480 ii with led is suitable for detection systems with different probe types such as hybridization probes or hydrolysis 5nuclease taqman probes. Intended use these instructions are intended for use of eurofins genescan lr, nr, umm kits on roche lightcycler 480 i and ii. Enter username and password when the login dialog box appears and click the button to proceed with the login. With a total of 6 detection channels, this compact cycler offers the highest flexibility and allows single color and multiplex testing.
The lightcycler 480 instrument ii is a rapid, platebased, thermal block cycler with integrated realtime, online detection capabilities. Dec 19, 2014 lightcycler 480 service software, free download by roche. This is an algorithm based on the kinetics of a pcr. Benefit from cuttingedge thermal block and optical technology that leads to excellent welltowell homogeneity and maximized detection sensitivity. The lightcycler 480 software allows the user to easily alternate between screens e. Information specific for single kits can be found in the individual manuals for the respective kits. This application can be used to edit and export the ts 480 information. We zoom out for a front view of the roche lightcycler 480 ii 384 pcr instrument, zoom in on software screen, showing software version, show connectivity, and lamp intensity. Lightcycler 480 realtime pcr system gene quantification. The lightcycler 480 system is a proven highperformance, mediumto highthroughput pcr platform that provides various methods for gene detection, gene expression analysis, genetic variation analysis, and array data validation.
Used roche lightcycler480 roche lightcycler480ii384 real. I have a problem with the analysis in lightcycler 480 software. Roche lightcycler 480 ii inquire about availability includes computer loaded with lightcycler 480 software release 1. Roche lightcycler whether your interest is in gene expression profiling or in detecting genetic variations, there is a member of the lightcycler system family offering the analytical performance and throughput you need for your research. The lightcycler 480 software uses unique roche algorithms for highly accurate and robust automated data analysis.
Start the lightcycler 480 software by doubleclicking the icon. Roche lightcycler 480 ii 96 pcr thermocycler 96silber block cycler cover dell latitude i5 running windows 10 with lightcycler 480 software ver1. For gene expression and gene dosage studies the highly flexible lightcycler 480 relative quantification software provides the user with more than one choice for relative quantification. The lightcycler 480 gene scanning software is intended for general laboratory use in combination with the lightcycler 480 instrument and lightcycler 480 software. Roche lightcycler 480ii384 pcr w thermocycler 384s. The new lightcycler 480 system includes sophisti cated software algorithms that generate data for absolute and relative quantification. Lightcycler software 4, free lightcycler software 4 software downloads. For more information, please consult chapter d, section 4. Lightcycler 480 gene scanning software roche life science. Roche lightcycler 480 ii 384 pcr thermocycler 384s block cycler cover dell latitude i5 running windows 7 pro with lightcycler 480 software ver1. I aborted my qpcr run lightcycler 480, roche in the cooling program because i. Used roche lightcycler 480ii384 roche lightcycler 480ii. The benchtop instrument is easily customizable to meet changing user requirements, and can be integrated into everyday use as a robotically controlled, automated high. I prepared the twostep cycling experiment with the following conditions.
The lightcycler480 system is a highperformance, medium to highthroughput pcr platform 96 or 384well plates that provides various methods for gene detection, gene expression analysis, genetic variation analysis, and array data validation. The roche lightcycler 480 ii 384 pcr instrument passed all tests and was found to be fully functional. For data analysis using the lightcycler 480 software, we highly recommend the use of the second derivative maximum analysis method available in this software. Comprehensive suite of tools for setup, run, and analysis of realtime pcr quantification and genotyping assays on the lightcycler 480 realtime pcr system. May 09, 20 roche lightcycler 480 ii pcr w thermocycler 384s bostonind 16949 duration. The roche lightcycler 480 system another breakthrough in realtime pcr analysis of gene expression and melting curvebased mutation analysis. Benefit from cuttingedge thermal block and optical technology that leads to excellent welltowell homogeneity and. The lightcycler 480 system is a highperformance, medium to highthroughput pcr platform 96 or 384well plates that provides various methods for gene detection, gene expression analysis, genetic variation analysis, and array data validation. Roche applied science lightcycler 480 realtime pcr system.
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